The Hollywood couple, who reportedly met on the set of comedy film Wanderlust, said the mutual decision was “lovingly made” at the end of last year.
Jennifer Aniston, 49, and Justin Theroux, 46, gave no reason for the split and said they intended to continue their “cherished friendship”.
They were married in a secret ceremony in Los Angeles in August 2015.
Jennifer Aniston and Justin Theroux had been together for four years before the wedding, which took place at their Bel Air mansion.
In a joint statement, the couple said they intended to remain friends: “We are two best friends who have decided to part ways as a couple, but look forward to continuing our cherished friendship.
“Normally we would do this privately, but given that the gossip industry cannot resist an opportunity to speculate and invent, we wanted to convey the truth directly.”
“Above all, we are determined to maintain the deep respect and love that we have for one another,” the statement added.
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