The decision to expel staff was made on July 29 , but President Putin has now confirmed the number who must go by September 1.
It brings staff levels to 455, the same as Russia’s complement in Washington.
This is thought to be the largest expulsion of diplomats from any country in modern history.
The number includes Russian employees of the US diplomatic missions across Russia.
Staff in the embassy in Moscow as well as the consulates in Ekaterinburg, Vladivostok and St. Petersburg are affected.
Vladimir Putin did strike a conciliatory note, saying he did not want to impose more measures, but also said he could not see ties changing “anytime soon”.
The president told Russian TV: “More than 1,000 people were working and are still working” at the US embassy and consulates, and that “755 people must stop their activities in Russia.”
Russia has also said it is seizing holiday properties and a warehouse used by US diplomats.
Vladimir Putin suggested he could consider more measures, but said: “I am against it as of today.”
The Russian president also noted the creation of a de-escalation zone in southern Syria as an example of a concrete result of working together.
However, in terms of general relations, Vladimir Putin added: “We have waited long enough, hoping that the situation would perhaps change for the better.
“But it seems that even if the situation is changing, it’s not for anytime soon.”
The new US sanctions were in retaliation both for Russia’s annexation of Crimea in 2014 and Russian interference in the US election.
In December, the Obama administration ordered the seizure of two Russian diplomatic compounds and expelled 35 Russian diplomats in response to alleged hacking of the US Democratic Party and Hillary Clinton’s campaign.
The new US sanctions on Russia were overwhelmingly approved by both houses of Congress despite objections from the White House.
US intelligence agencies believe Russia tried to sway the election in favor of President Donald Trump and now there are several investigations looking into whether anyone from his campaign helped.
Russia has always denied interfering and Donald Trump insists there was no collusion.
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