The former New England Patriots tight end hanged himself at the Souza Baranowski Correctional Center and was pronounced dead later at hospital.
Aaron Hernandez, 27, was serving a life sentence for killing a man who was dating his fiancée’s sister.
Last week the former NFL star was acquitted of a drive-by double murder prosecutors said began over an argument about a spilled drink.
Aaron Hernandez had cried in court as that verdict was read, saying he was “very happy”.
His death comes on the day members of his former team visit President Donald Trump at the White House to be commended for their Super Bowl victory over Atlanta in February.
According to Massachusetts Department of Correction, Aaron Hernandez had tried to block the door to his single cell before hanging himself.
Lifesaving techniques were performed on him and he was taken to UMASS Leominster hospital where he was pronounced dead at 04:07 local time.
State police are on the scene and Aaron Hernandez’s next of kin have been notified, officials said.
State Department of Correction spokesman Christopher Fallon said in a statement that he was not aware a suicide note had been written and that officials had not been concerned that Aaron Hernandez would take his life.
Although Aaron Hernandez was acquitted of the double murder last week, he was found guilty of illegally possessing a firearm and the judge added five years to his sentence.
He had been accused of the fatal shooting of Daniel de Abreu and Safiro Furtado outside a Boston nightclub in 2012, one month before he signed a $40 million contract extension with the Patriots.
In April 2015, Aaron Hernandez had been found guilty of the first-degree murder of his friend, another American footballer, the semi-professional Odin Lloyd, in 2013.
Odin Lloyd’s body was found with six bullet wounds less than a mile from Aaron Hernandez’s home.
Within hours of his arrest for the 2013 shooting, the Patriots sacked Aaron Hernandez, considered one of the top tight ends playing the game.
Aaron Hernandez was given a life sentence without parole.
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