Dozens of others remain in critical condition after the fire in the Colectiv club, apparently caused by fireworks set off by a heavy metal band.
According to officials, it is possible the number of fatalities could double.
The news came as 12,000 people marched silently through the Romanian capital in memory of the victims.
People carried flowers and lit candles at a memorial outside the nightclub.
Romanian President Klaus Iohannis has said safety regulations seem to have been ignored at the club.
The fire broke out at about 22:30 local time on October 30 at the Colectiv club, which was hosting a free rock concert by the band Goodbye to Gravity. Up to 400 people are thought to have been inside.
Survivors say the fireworks caused the ceiling and a pillar to catch fire and produce heavy smoke.
A stampede for the exit followed. More than 140 people are being treated in hospital, some with severe burns.
On November 1 officials said all of the dead had been identified.
Romania has declared three days of national mourning after the tragedy.
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