The Republican presidential frontrunner said: “If I win, they’re going back.”
It marks a reversal in policy – earlier this month Donald Trump told Fox News the US should take in more refugees.
A refugee crisis has gripped parts of Europe and the US has pledged to take 10,000 refugees from Syria in 2016.
Half a million people have crossed the Mediterranean into Europe in 2015, with the largest number from Syria, where 250,000 people have been killed in a civil war.
On September 30, Donald Trump told an audience at Keene High School: “I hear we want to take in 200,000 Syrians. And they could be – listen, they could be ISIS [Islamic State].”
Describing them as a “200,000-man army”, the billionaire later added: “I’m putting the people on notice that are coming here from Syria as part of this mass migration, that if I win, if I win, they’re going back.”
Donald Trump has made immigration a central plank of his election campaign, pledging to build a wall on the southern border.
He was harshly criticized after saying undocumented Mexican immigrants were “bringing drugs, they’re bringing crime, they’re rapists”.
Donald Trump’s latest comments about sending Syrians home are more in line with his hardline immigration policy, although at odds with what he said earlier this month.
Asked whether he thought some of the migrants travelling into Europe should be allowed in the US, the business mogul said: “I hate the concept of it, but on a humanitarian basis, with what’s happening, you have to.”
Donald Trump blamed President Barack Obama for the crisis and added: “It’s living in hell in Syria. They are living in hell.”
The US has allowed 1,500 Syrians to re-settle since the start of the conflict four years ago.
A number of Democrats, including Hillary Clinton, have urged the US to increase the number of Syrians from 10,000 to 65,000.
Secretary of State John Kerry has pledged to take more refugees worldwide, raising the yearly cap from 70,000 to 85,000 in 2016 and to 100,000 in 2017.
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