Scientists made the connection on an expedition to the boy’s village, Meliandou.
They took samples and chatted to locals to find out more about Ebola’s source.
The team’s findings are published in EMBO Molecular Medicine.
Meliandou is a small village of 31 houses.
It sits deep within the Guinean forest region, surrounded by towering reeds and oil palm cultivations – these are believed to have attracted the fruit bats carrying the virus passed on to Emile Ouamouno.
During their four-week field trip in April 2014, Dr. Fabian Leendertz, from the Robert Koch Institute in Germany, and colleagues found a large tree stump situated about 165ft from Emile Ouamouno’s home.
Villagers reported that children used to play frequently in the hollow tree.
Emile Ouamouno – who died of Ebola in December 2013 – used to play there, according to his friends.
The villagers said that the tree burned on March 24, 2014 and that once the tree caught fire, there issued a “rain of bats”.
A large number of these insectivorous free-tailed bats – Mops condylurus in Latin – were collected by the villagers for food, but disposed of the next day after a government-led ban on bushmeat consumption was announced.
While bushmeat is thought to be a possible source of Ebola, the scientists believe it didn’t trigger the outbreak.
Instead, it was Emile Ouamouno’s exposure to the bats and their droppings as he played with his friends in the hollowed tree.
The scientists took and tested ash samples from the tree and found DNA traces that were a match for the animals.
While they were unable to test any of the bushmeat that the villagers had disposed of, they captured and tested any living bats they could find in and around Meliandou.
No Ebola could be detected in any of these hundred or so animals, however.
But previous tests show this species of bat can carry Ebola.
Dr. Fabian Leendertz and his colleagues say this must be a pretty rare occurrence though.
He said: “That is also obvious when you think about how many tonnes of bat meat is consumed every year.
“If more bats carried the virus, we would see outbreaks all the time.”
Dr. Fabian Leendertz says it is vital to find out more about the bats.
“They have moved into human settlements. They do not just live in the trees but also under the roofs of houses in the villages.
“The Ebola virus must jump through colonies from bat to bat, so we need to know more.”
He added: “We need to find ways to live together with the wildlife. These bats catch insects and pests, such as mosquitoes. They can eat about a quarter of their body weight in insects a day.
“Killing them would not be a solution. You would have more malaria.”
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