The Marvel release extended its run at the top of the movie chart, taking $16.3 million across the Labor Day weekend.
Guardians of the Galaxy has now taken a total of $275 million so far.
Captain America is the second biggest film of 2014 to date, followed by the Lego Movie.
Transformers movie is at number four on the annual list.
Over the Labor Day weekend, Guardians of the Galaxy kept Teenage Mutant Ninja Turtles in second spot, with If I Stay remaining at three with takings of $9.3 million.
Pierce Brosnan action flick The November Man entered at six.
The release took a modest $7.7 million and was outperformed by thriller As Above, So Below, which made its debut at number four.
Comedy Let’s Be Cops was fifth.
Guardians of the Galaxy entered the chart at number one at the beginning of August, before temporarily losing its crown to Teenage Mutant Ninja Turtles.
The space adventure stars Zoe Saldana and Chris Pratt, and also features the voices of Bradley Cooper and Vin Diesel.
A sequel to the Marvel Comics adaptation is expected to be released in 2017.
Despite Guardians of the Galaxy ‘s success, the summer box office in North America is down on last year.
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