Glen Campbell, 78, revealed he had been diagnosed with the disease in 2011.
Kim Campbell said doctors had persuaded her to discontinue care at the family’s home, angering the country music star’s daughter Debby.
“I am his wife and no one wants him home more than me but I must do what is in his best interest,” Kim Campbell told AP.
“It is crushingly sad to see him afflicted with Alzheimer’s but indulging those feelings does not help him,” added Kim Campbell, the singer’s fourth wife of 32 years.
Talking about the care her husband is now receiving, Kim Campbell said: “He has activities and therapies to stimulate him and help him experience daily moments of success.
“His life is filled with love and laughter and he is being cared for round the clock by people who specialize in Alzheimer’s care and happen to adore him.”
Debby Campbell, one of the singer’s eight children, told Country Weekly magazine last week that she was unhappy with the decision to move her father into care.
She also said Glen Campbell had not been receiving enough attention from family members in Nashville, where the music star lives.
However, Kim Campbell countered by saying she spent time with her husband every day and that two of his children who live in Nashville visit weekly.
She also said she organized activities for the artist.
Glen Campbell issued two albums and went on a world tour following his diagnosis.
At the time, his wife said the tour was a way to help her husband combat the disease and spend time with his family members, including Debby, who was in his band and travelled with him.
Glen Campbell received a Lifetime Achievement Grammy in 2012. He has also been named Male Vocalist of the Year by the Country Music Association (CMA) and the Academy of Country Music (ACM).
In 2005, Glen Campbell was inducted into the Country Music Hall of Fame.
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