The White House said the move was a response to “Russia’s continued illegal intervention in Ukraine”.
Those targeted include Igor Sechin, head of oil giant Rosneft, and Sergei Chemezov of the hi-tech firm Rostec.
The announcement comes after the mayor of Kharkiv, a city in eastern Ukraine, was shot and critically wounded.
Hennadiy Kernes was recovering after an operation to repair damage to the chest and abdomen, but his life remained in danger, his office said.
Monday also saw pro-Russian separatists, whom Western nations accuse Moscow of supporting, seize a local government building in Kostyantynivka, a town in the eastern Donetsk region.
In Donetsk itself, pro-Russian activists armed with clubs and chains attacked a pro-unity rally, correspondents and eyewitnesses said. A number of people were injured in the clash.
Separatists were also continuing to detain about 40 people in the town of Sloviansk, including journalists, pro-Kiev activists and seven military observers linked to the Organization for Security and Co-operation in Europe (OSCE) as well as three members of Ukraine’s security service, officials in Kiev said.
At a special meeting of the OSCE’s permanent council in Vienna, Russian ambassador Andrei Kelin said Moscow was taking “steps” to secure the observers’ release. But the area around Sloviansk was very tense and it had been “extremely irresponsible” to send them there, he added.
The US and EU first imposed visa bans and asset freezes on a number of senior Russian officials and companies after Moscow annexed Crimea from Ukraine last month.
On Thursday, the White House said it was adding to its sanction list “in response to Russia’s continued illegal intervention in Ukraine and provocative acts that undermine Ukraine’s democracy and threaten its peace, security, stability, sovereignty, and territorial integrity”.
It accused Russia of “doing nothing to meet the commitments it made” at a meeting with Ukraine, the US and EU in Geneva on April 17, which it said had included refraining from violence or provocative acts.
Russian Deputy Foreign Minister Sergei Ryabkov said Moscow’s response would be “painful for Washington”.
He told the Interfax news agency that the Kremlin was “disgusted” by the sanctions, which he said showed the White House had “completely lost touch with reality”.
Most of the 17 companies targeted are linked to Arkady and Boris Rotenberg and Gennady Timchenko – individuals targeted in the previous sanctions list.
The latest measures also target some hi-tech exports that “could contribute to Russia’s military capabilities”.
Diplomats in Brussels also said European Union governments had reached a preliminary agreement to impose asset freezes and visa bans on another 15 people as part of expanded sanctions on Russia over its actions in Ukraine. The names are expected to be released on Tuesday, although more could still be added.
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