The Way Is Love was pieced together by Wesley, Alex and Roy Orbison Jr. after finding an old vocal recording on a home tape machine.
They reclaimed it using technology which was specifically developed to accomplish the task.
The track will appear on the 25th anniversary edition of Roy Orbison’s final album, Mystery Girl.
Roy Orbison died of a heart attack in December 1988, aged 52, two months before the record was originally released.
A total of nine previously unheard songs will be added to the deluxe edition, which is due on May 20.
The Orbison brothers added new instrumental recordings to The Way Is Love with the help of another offspring of a music legend, co-producer John Carter Cash.
Mystery Girl included contributions from Tom Petty, Bono and the Edge of U2, and Elvis Costello.
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