The Egypt’s ousted Islamist president was taken to the court in Cairo by helicopter from a prison in Alexandria, Mena news agency reports.
Mohamed Morsi, Egypt’s first freely-elected president, was deposed by the military in July 2013 after mass protests against his rule.
He is now facing four separate criminal trials on various charges.
Also on Tuesday, the interior ministry said that a ministry official, named in local media as General Mohammed Saeed, was shot dead on his way to work.
The killing comes amid a string of militant attacks on security services in recent days, and hours after Egypt’s top military body gave its backing for army chief Field Marshal Abdel Fattah al-Sisi to run for president.
Supporters of Field Marshal Abdel Fattah al-Sisi have gathered outside the building, but no pro-Morsi supporters have appeared.
Mohamed Morsi’s Muslim Brotherhood has been declared a terrorist organization and authorities have punished any public showing of support for it.
Another 130 people are also facing charges in the prison break trial, but many of the defendants are currently on the run.
Mohamed Morsi stands accused of organizing a mass breakout from the Wadi al-Natrun prison during the 2011 uprising that toppled President Hosni Mubarak, as well as the murder of prison officers.
He is appearing in a sound-proofed glass box during the trial and will only be permitted to speak after raising his hand.
State television reported that the defendants were shouting inside the glass box and were making the four-finger “Rabaa” protest sign, but could not be heard.
When he first appeared in court in November in a separate trial, Mohamed Morsi chanted slogans against the current government and the court. He also refused to recognize the court’s legitimacy or put on the required prison uniform.
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