Lady Gaga will star alongside the puppets in a 90-minute special on American network ABC.
Lady Gaga & The Muppets’ Holiday Spectacular will air on November 28 for Thanksgiving.
Elton John and Joseph Gordon-Levitt are among the other guests who will be a part of the show.
It’s the second time Lady Gaga, 27, has been part of the ABC’s holiday special.
“I was so excited when ABC called me about doing a holiday special this year,” said Lady Gaga.
“I knew it just wouldn’t be a complete night of laughter and memories without The Muppets!”
In 2009, Kermit the Frog was Lady Gaga’s date to the MTV Video Music Awards (VMAs).
“Can’t wait to see the gang again and I hope Miss Piggy’s still not mad about Kermit. We’re just friends!”
In a statement from ABC, Kermit the Frog said: “What an incredible opportunity!”
“We have a long history with Ms. Gaga, so we jumped at the chance to do a holiday special with her.”
Lady Gaga also wore a dress made of Kermit the Frog toys on German TV in the same year as her appearance with him at the VMAs.
“We won’t even mind if she mistakes some of the Muppets for pieces of her wardrobe, again,” added Kermit.
Lady Gaga is also set to perform at the first YouTube Music Awards with Eminem and Arcade Fire, among others.
The singer will appear on Sunday’s (October 27) X Factor results show.
It was announced that Lady Gaga will perform Venus from her upcoming third studio album Artpop, which is scheduled to be released in November.
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