Organizers of Go Go Gorillas event were contacted by Queen’s manager Jim Beach on behalf of the Freddie Mercury estate.
The estate claimed the suit “worn” by the gorilla breached copyright.
It asked the Freddie “Radio Go Go” Gorilla sculpture, which Norfolk artist Mik Richardson took three days to create, to be “taken off the streets”.
The sculpture was removed from Millennium Plain, outside The Forum, at 11:00 BST.
Mik Richardson, 50, of Aylsham, Norfolk, said the decision to order its removal was “absolutely shocking”.
He said: “It’s dreadful. It’s petty, really. The night I was told I couldn’t sleep.”
Mik Richardson was paid £800 ($1,250) to design and paint the gorilla.
“I’m a mural artist and I have to be very careful about copyright,” he said.
“I didn’t copy the suit exactly. I alter enough so that it’s fan art, rather than a copy of it.”
The “Radio Go Go” gorilla is one of 53 life-size gorillas decorated by Norfolk artists and displayed on the streets of Norwich over the summer.
An additional 67 baby gorillas, painted at local schools, made up the 120-strong public art trail.
The Freddie Mercury estate contacted Wild in Art, the company that supplied the gorilla glass-fibre canvases, according to director Charlie Langhorne.
“They just said that they own the copyright on the suit and asked us to change it,” Charlie Langhorne said.
“That’s being sorted. To save any bother we will change it.
“We do quite a few bits and pieces for them but it’s no great shakes.
“We would rather not have to do it but it’s not the end of the world.”
He said the gorilla would be repainted with a “new and exciting design” and should be back in place within 10 days.
“People in Norwich will have a new gorilla to enjoy soon,” he said.
Martin Green of Break, one of two charities that will benefit from the auction of the gorillas once the exhibition is over, said: “It’s a disappointing position they have put us in.
“Freddie is one of our most popular gorillas on the trail and now we’ve got to remove him from the streets.
“It’s a sad story, but the positive news is that we’ll end up with another gorilla on the streets.”
The Born Free Foundation, which counts Queen guitarist Brian May among its celebrity supporters, was also set to benefit from the October auction of the Freddie Mercury gorilla to help support conservation projects in the Congo.
A “Freddie Lion” had previously been commissioned for a Pride of Cape Town art trail in May.
A spokesman for the Freddie Mercury estate said it would “not be making any comment”.
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