Mega, a web-based service that lets people upload and store files of any kind, is a sequel to the Megaupload system that was shut down last January.
Police raids on the offices and home of Kim Dotcom led to the closure of Megaupload.
The Mega site went online at dawn on Sunday, with Kim Dotcom due to hold a gala at his New Zealand mansion later.
Kim Dotcom has said the new site complies with the law and warned that attempts to take it down would be futile.
“This is not some kind of finger to the US government or to Hollywood,” Kim Dotcom told Reuters on Saturday.
“Legally, there’s just nothing there that could be used to shut us down. This site is just as legitimate and has the right to exist as Dropbox, Boxnet and other competitors.”
Hours after the site was launched, Kim Dotcom tweeted that it had received 250,000 user registrations, although limited server capacity meant Mega was unreachable to many.
In a series of earlier tweets Kim Dotcom said every customer would have 50 gigabytes of free storage – far more than is offered by rival services such as Dropbox or Microsoft’s SkyDrive.
Mega will be encrypted so only those who upload data have access to it.
Data is also being held in the cloud to make it easy for users to get and share files.
The 2012 raids on Megaupload were carried out because, said US law enforcement, many users of Megaupload were engaged in pirating content and illegally sharing it.
They accused Kim Dotcom and other managers at Megaupload of profiting from piracy.
KIm Dotcom has rebuffed the accusations and is fighting a legal battle to stay in New Zealand from where he ran Megaupload.
A hearing on whether he is can be extradited to the US is due to be held in March.
The case has generated controversy in New Zealand over the way the police and intelligence services gathered evidence before the raid and won an apology to Kim Dotcom from the country’s prime minister.
KIm Dotcom has also won support from prominent computer pioneers such as Apple co-founder Steve Wozniak.
The raid on Megaupload put 25 petabytes of data uploaded to it by its 50 million members into a legal limbo.
In one message, Kim Dotcom said he was working with lawyers and the Electronic Frontier Foundation, which campaigns on digital rights issues, to get access to that seized data and return it to users.
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