Lady Gaga, 26, had just begun to strut down a set of stairs on to the main stage area when her sickness kicked in.
The pop performer, who was dressed in a typically revealing outfit, halted at the top of the steps and was seen leaning over the edge with her back to the audience.
After following the recent example of Justin Bieber, by throwing up, she attempted bravely to continue with her dance routine as she began to perform of Edge Of Glory.
However, Lady Gaga was unable to carry with the routine without stopping again to vomit over the barrier behind her.
Ever the professional Lady Gaga valiantly did more dance moves in between throwing up even more while her male dancer tried his best to obscure the view by staying in front of her.
Lady Gaga held up her own hair as she avoided getting any mess on herself before standing erect and bouncing across the stage to entertain the audience.
It is not the first time Lady Gaga has fallen ill on stage as she was forced to halt a concert in Romania back in August to vomit off camera.
When she returned to the stage she said: “I went backstage and vomited, and I did not want you to see this… It happens to me sometimes.”
Things only got worse for the singer as some of the 25,000 strong crowd poked fun at her over her recent 25 lbs weight gain.
Lady Gaga was showered with sausages but took the jibe in her stride as she tweeted after: “SPAIN, you took the p*** outta me. Thats some hard bodied pop stuff. I’ll never forget tonight Barcelona. FOREVER.”
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