Danny DeVito, 67, married former Cheers actress Rhea Perlman, 64, in 1982 and they have three grown up children, Daniel, 25, Grace, 27, and Lucy, 29.
The pair worked together on the classic Seventies sitcom Taxi and later appeared in the 1996 comedy film Matilda.
Danny DeVito and Rhea Perlman met when she went to see off-Broadway play, The Shrinking Bride, which he was appearing in.
The couple founded Jersey Films, a production company that produced films including Pulp Fiction and Garden State.
A representative for Danny DeVito confirmed the split to but had no other comment.
Danny DeVito was most recently seen on stage for a two-month run of The Sunshine Boys at the Savoy Theatre in London’s West End this summer.
He earned positive reviews for his performance as Willie Clark, a sharp-tongued curmudgeon who has a bittersweet reunion with his former vaudeville partner.
Meanwhile Danny DeVito may soon be headed back to the big screen alongside former Twins co-star Arnold Schwarzenegger for a sequel to their Eighties hit.
He told fans on the Today show: “We have the desire to do it. What we need is the screenplay… We want to do something really good, so we’re waiting.”
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