In an email sent to the 10,000 members of the public participating in the 27 July event, Danny Boyle asked them to “stay virtuous” and “protect the show”.
“Many of you have been dismayed by the media scrutiny on the show,” he wrote.
“I am sorry that, despite our best efforts, we appear to be unable to stop these stories appearing in the press.”
At a media briefing last month, the Oscar-winning director revealed the ceremony would feature a recreation of the “British countryside” featuring country scenes and farmyard animals.
In the weeks that followed, several newspapers published follow-up stories revealing additional, unsanctioned information about the event.
“Some of you have asked why we agreed to the two media briefings we have done when <<open season>> continues on trying to reveal every aspect of our work to the public ahead of 27 July,” wrote Danny Boyle in his email.
“We thought they would be a good way to satisfy the media’s curiosity about our show but, in the case of certain papers, it hasn’t quenched their desire to be the first to reveal every detail possible.”
London 2012 organizing committee chairman Lord Coe made similar comments on Twitter at the weekend asking for details to be kept secret.
“Share the frustration of volunteer performers and the public at Opening Ceremony being unofficially trailed. Let’s #savethesurprise,” he said.
One billion people worldwide are expected to watch the opening ceremony, which will feature music by electronic duo Underworld and a “harmonically-tuned” bell weighing 23 tons.
Cast rehearsals continue at the Olympic stadium in east London, which has been fitted with a million-watt sound system.
Cast members have been asked to sign and abide by a non-disclosure agreement.
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