Scientists estimate he was about 100 years old.
Park officials said they would carry out a post-mortem to determine the cause of his death.
With no offspring and no known individuals from his subspecies left, Lonesome George became known as the rarest creature in the world.
For decades, environmentalists unsuccessfully tried to get the Pinta Island tortoise to reproduce with females from a similar subspecies on the Galapagos Islands.
Park officials said the tortoise was found dead in his corral by his keeper of 40 years, Fausto Llerena.
While his exact age was not known, Lonesome George was estimated to be about 100, which made him a young adult as the subspecies can live up to an age of 200.
Lonesome George was first seen by a Hungarian scientist on the Galapagos island of Pinta in 1972.
Environmentalists had believed his subspecies (Chelonoidis nigra abingdoni) had become extinct.
Lonesome George became part of the Galapagos National Park breeding programme.
After 15 years of living with a female tortoise from the nearby Wolf volcano, Lonesome George did mate, but the eggs were infertile.
He also shared his corral with female tortoises from Espanola island, which are genetically closer to him than those from Wolf volcano, but Lonesome George failed to mate with them.
He became a symbol of the Galapagos Islands, which attract some 180,000 visitors a year.
Galapagos National Park officials said that with George’s death, the Pinta tortoise subspecies has become extinct.
They said his body would probably be embalmed to conserve him for future generations.
Tortoises were plentiful on the Galapagos islands until the late 19th century, but were later hunted for their meat by sailors and fishermen to the point of extinction.
Their habitat furthermore suffered when goats were introduced from the mainland.
The differences in appearance between tortoises from different Galapagos islands were among the features which helped the British naturalist Charles Darwin formulate his theory of evolution.
Some 20,000 giant tortoises of other subspecies still live on the Galapagos.
JyOLK3QVprEThe US House Ethics Committee has voted to release its report on former Republican Representative…
ABC News has agreed to pay $15 million to President-elect Donald Trump to settle a…
South Korea’s parliament has voted to impeach President Yoon Suk Yeol over his failed attempt…
Israeli war planes have carried out more than 100 air strikes in Syria on December…
President-elect Donald Trump has threatened to impose 100% tariffs on the BRICS countries if they…
Syrian troops have withdrawn from the city of Aleppo following an offensive by rebels opposed…