Cuba denies the existence of any sonic attack against US embassy staff in Havana and says the claims are a “political manipulation” aimed at damaging bilateral relations.
Cuban Foreign Minister Bruno Rodriguez dismissed allegations of any kind of attack as “totally false”.
The US said nearly two dozen embassy personnel had health problems after the alleged attacks and cut its staff as a result.
Reports suggest sonic attacks were to blame, but nothing has been proven.
The US has not blamed Cuba for the alleged attacks, and the Cuban government has previously denied targeting embassy staff.
Meanwhile, the US expelled 15 Cuban diplomats, saying that Havana had failed to protect its employees, but Cuba said the move was “unjustified”.
The US government also suspended visa processing in Cuba indefinitely.
Speaking in Washington at a meeting of Cubans living in the US, Bruno Rodriguez said the allegations have caused a “serious deterioration in the relationship between both governments and both countries”.
“It’s unacceptable and immoral, from the point of view of the Cuban government, for people to be harmed by a difference between governments,” the minister added.
The reported health problems ranged from mild brain trauma and deafness to dizziness and nausea.
Secretary of State Rex Tillerson said in a statement on October 3: “The decision was made due to Cuba’s failure to take appropriate steps to protect our diplomats in accordance with its obligations under the Vienna Convention. This order will ensure equity in our respective diplomatic operations.”
At least 21 people working at the US’s embassy in Havana have reported health problems, ranging from mild brain trauma and deafness to dizziness and nausea.
Earlier reports suggested sonic attacks were to blame, but nothing has been proven.
Havana denies targeting embassy staff, and the US has not blamed the Cuban government for the suspected attacks.
Rex Tillerson added in his latest statement: “We continue to maintain diplomatic relations with Cuba, and will continue to co-operate with Cuba as we pursue the investigation into these attacks.”
Two Cuban diplomats have been expelled by Washington after US embassy staff in Havana suffered mysterious physical symptoms, the US state department announced.
It was not immediately clear what had happened, but spokeswoman Heather Nauert said there were no “definitive answers about the source or cause”.
One government official quoted by Reuters said that some staff had suffered hearing loss.
Cuba’s foreign ministry announced it was investigating the allegations.
Cuba called the expulsion of its staff unjustified but said it was willing to co-operate with Washington to clarify what had happened.
The ministry said: “Cuba has never, nor would ever, allow the Cuban territory to be used for any kind of action against accredited diplomats or their families.”
It was suggested the hearing loss could have been caused by some kind of sonic device placed outside the diplomats’ homes, emitting inaudible sound waves that can cause deafness.
Heather Nauert said staff began complaining of the strange symptoms late last year.
While they were not life-threatening, she revealed that a number of people had been brought home to the US as a result.
Heather Nauert said the government was taking it “very seriously, and there is an investigation currently under way”.
It is understood that the two expelled Cuban diplomats left Washington DC in May..
The US and Cuba only re-established ties in 2015, following 50 years of hostilities between the two countries.
Fidel Castro has published an open letter to Cubans in which he makes no mention of the historic reopening of the US embassy in Havana.
The former Cuban leader instead criticizes American foreign and economic policies since World War Two and accuses the US of owing Cuba millions of dollars.
The letter was published to mark Fidel Castro’s 89th birthday.
The US embassy will be reopened in Havana on August 14, with Secretary of State John Kerry attending.
Fidel Castro said the US owed Cuba money because of the trade embargo the US imposed on the communist-run island in 1960.
Cuba says the embargo – which it calls a blockade – is hugely damaging to its economy.
The letter says relations will only be fully restored once it is lifted.
Three marines who lowered the American flag for the last time on January 4, 1961, will raise it again during Friday’s ceremony in Havana.
They are now retired and in their late 70s.
“I’m gonna love seeing that flag go back up,” said former marine Jim Tracy, 78, on a State Department video.
Cuba reopened its embassy in Washington last month.
In his birthday letter published in state newspaper Granma, Fidel Castro says Cuba is committed to “good will and peace in our hemisphere” but adds: “We will never stop fighting for the peace and welfare of all human beings, regardless of the color of their skin and which country they come from.”
Fidel Castro led his country from the Cuban Revolution, in 1959, until 2006, when he stood down because of undisclosed health problems.
He passed on power to his younger brother, Raul Castro, who embarked on a number of economic reforms.
After Raul Castro and President Barack Obama announced in December that Cuba and the US had agreed to restore diplomatic relations, it took Fidel Castro more than a month to express lukewarm approval for the historic reconciliation.
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