The internet movie and television streaming service said its content, such as series House of Cards and Orange is the New Black, will be available to anyone with international payment cards.
On January 15, the US announced new rules that ease long-running sanctions against Cuba.
A trade embargo has been in place since 1962.
MasterCard and American Express have both said they will launch operations in Cuba soon.
Last month, Netflix said its international expansion was proceeding ahead of schedule.
The company said it plans to expand to 200 countries in the next two years, and to earn “material profits” from its operations overseas by 2017.
“We are delighted to finally be able to offer Netflix to the people of Cuba, connecting them with stories they will love from all over the world,” said Netflix co-founder and CEO Reed Hastings in a statement.
“Cuba has great filmmakers and a robust arts culture and one day we hope to be able to bring their work to our global audience of over 57 million members.”
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