A statement on the Ghana Football Association website said both players had “been suspended indefinitely”.
The statement added Kevin-Prince Boateng had used “insults targeted at coach Kwesi Appiah” and said Sulley Muntari was guilty of an “unprovoked physical attack on an executive committee member”.
The news comes just hours before Ghana take on Portugal on Thursday.
Ghana still has an outside chance of qualifying for the knockout phase of the tournament.
The Ghana FA statement said the incident involving AC Milan’s Sulley Muntari took place on Tuesday and named Moses Armah, a member of the team management, as the man said to have been assaulted by the 29-year-old.
And Schalke 04 player Kevin-Prince Boateng, 27, was said by the Ghana FA to have shown “no remorse for his actions”, which took place during the team’s training session in Maceio on Tuesday.
Ghana’s World Cup campaign has already been blighted by a dispute over players’ appearance fees.
On Wednesday, the national government sent in excess of $3 million in a chartered plane to Brazil to pay the squad following their threat to boycott their final group match against Portugal if they did not receive their money.
To reach the last 16, Ghana need to beat Portugal and hope there is a winner of the match between Germany and US – although Ghana would also need to overtake their rivals’ superior goal difference.
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